• Molecular identification of single larvae of Trichinella at the species or genotype level
• Morphological identification of larvae or adults of Trichinella
• Immunohistochemical identification of Trichinella larvae in muscle samples fixed in formalin or paraffin embedded
• Detection of anti-Trichinella antibodies in human sera by validated ELISA and Western bot
• Detection of anti-Trichinella antibodies in pig sera by validated ELISA and Western bot
• Detection of anti-Trichinella antibodies in other animal species by validated ELISA and Western blot

For more information on molecular identification of Trichinella taxa read the following publications:

• Pozio E, La Rosa G. 2010. Trichinella. In: Liu D. (Ed.), Molecular detection of Foodborne Pathogens. CRC Press, Taylor & Francis Group, Boca Raton, USA, pp. 851-863.

For more information on serology read the following publications:

• Bruschi F, Gómez-Morales MA, Hill DE, 2019. International Commission on Trichinellosis: Recommendations on the use of serological tests for the detection of Trichinella infection in animals and humans. Food Waterborne Parasitol. 14:e00032.
• Gómez-Morales MA, Ludovisi A, Amati M, Cherchi S, Tonanzi D, Pozio E., 2018. Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot. Parasit Vectors. 11:631.
• Gómez-Morales MA, Selmi M, Ludovisi A, Amati M, Fiorentino E, Breviglieri L, Poglayen G, Pozio E. 2016. Hunting dogs as sentinel animals for monitoring infections with Trichinella spp. in wildlife. Parasit Vectors. 9:154.
• Gómez-Morales MA, Ludovisi A, Amati M, Pozio E. 2015. Candidates for reference swine serum with anti-Trichinella antibodies. Vet Parasitol. 208:218-24.
• Gómez-Morales MA, Ludovisi A, Amati M, Bandino E, Capelli G, Corrias F, Gelmini L, Nardi A, Sacchi C, Cherchi S, Lalle M, Pozio E. 2014. Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa). Parasit Vectors. 7:171.
• Gómez-Morales MA, Ludovisi A, Amati M, Blaga R, Zivojinovic M, Ribicich M, Pozio E. 2012. A distinctive Western blot pattern to recognize Trichinella infections in humans and pigs. Int J Parasitol. 42:1017-23.
• Gómez-Morales MA, Ludovisi A, Amati M, Cherchi S, Pezzotti P, Pozio E. 2008. Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis. Clin Vaccine Immunol. 15:1723-9.

Reference material available at the ITRC

• Reference strains in vivo
• Larvae of reference strains in ethyl alcohol
• Crude antigens of Trichinella spiralis
• Excretory/secretory antigens of Trichinella spiralis
• Reference sera from Trichinella-positive humans and animals

For more information contact us by email giuseppe.larosa@iss.it, mariaangeles.gomezmorales@iss.it